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Genetic analysis of dermatophilus spp. using multilocus enzyme electrophoresis
D J Trott1, A M Masters, J M Carson
1School of Veterinary Studies, Murdoch University, Western Australia.
Zentralblatt Fur Bakteriologie : International Journal of Medical Microbiology
|January 1, 1995
Summary
Multilocus enzyme electrophoresis revealed significant genetic diversity within Dermatophilus congolensis, identifying eight distinct electrophoretic types (ETs). Isolates from Chelonids represent a distinct clone, supporting their classification as a new species.
Area of Science:
- Microbiology
- Genetics
- Veterinary Science
Background:
- Dermatophilus congolensis causes various skin infections in animals.
- Previous studies suggested Chelonid isolates may represent a distinct species.
Purpose of the Study:
- To genetically characterize Dermatophilus congolensis isolates using multilocus enzyme electrophoresis.
- To investigate the genetic relationship between Chelonid isolates and other D. congolensis strains.
Main Methods:
- Multilocus enzyme electrophoresis (MEE) was performed on 41 Dermatophilus congolensis isolates.
- Genetic variation was assessed across 16 enzyme loci.
- Genetic distances were calculated to determine relationships between isolates.
Main Results:
- Eight distinct electrophoretic types (ETs) were identified among the 41 isolates.
- Chelonid isolates formed a distinct clone (ET 1), separate from other D. congolensis isolates.
- Two major divisions (I and II) were observed within the D. congolensis cluster, correlating with hemolytic patterns.
- Genetic diversity within the D. congolensis population was significant, with no strong correlation between host species/geographic origin and genetic relatedness.
Conclusions:
- Chelonid isolates of Dermatophilus are genetically distinct and likely represent a new species.
- Significant genetic diversity exists within Dermatophilus congolensis, with distinct electrophoretic types and divisions observed.
- MEE is a valuable tool for differentiating Dermatophilus isolates and complements DNA restriction endonuclease analysis.