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The influence of cations and ionic strength on actin polymerization in the presence/absence of alpha-actinin
1Technical University of Munich, Garching, FRG.
Biochemistry and Molecular Biology International
|January 1, 1995
Abstract:
The presence of alpha-actinin has little influence on polymerizing actin with regards to the lag phase, rate and amplitude. Rising KCl or MgCl2 concentrations parallel an increase in polymerization rate and a decrease in lag phase for both protein species. The observed overall smaller actin fluorescence amplitudes in the presence of alpha-actinin is due to a higher ratio of free G-actin to F-actin at steady state.