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T-lymphocyte Ca2+ signalling and proliferative responses during sepsis
M A Choudhry1, S Ahmad, K D Thompson
1Department of Physiology, Loyola University of Chicago, Stritch School of Medicine, Maywood 60153, USA.
Shock (Augusta, Ga.)
|April 1, 1994
Summary
Sepsis impairs T-lymphocyte function, decreasing intracellular calcium (Ca2+) signaling and proliferation responses to concanavalin A (Con A). This T-cell dysfunction may reduce the host
Area of Science:
- Immunology
- Cellular Biology
- Sepsis Research
Background:
- T-lymphocyte alterations are linked to burn and traumatic injuries.
- Sepsis can profoundly impact immune cell function and host defense.
Purpose of the Study:
- To investigate the effects of sepsis on T-lymphocyte intracellular calcium (Ca2+) signaling and proliferation.
- To evaluate the regulation of T-cell responses by concanavalin A (Con A) in a septic rat model.
Main Methods:
- Sepsis was induced in rats using Escherichia coli and Bacteroides fragilis.
- Intracellular Ca2+ ([Ca2+]i) in T-lymphocytes was measured using Fura-2 and microfluorophotometry.
- T-cell proliferation was assessed after 72-hour culture with Con A.
Main Results:
- Septic rat T-lymphocytes showed a significantly decreased Ca2+ response to Con A on days 1 and 2 post-implantation.
- A significant reduction in Con A-mediated T-cell proliferation was observed on day 2 in septic rats compared to controls.
- The decrease in T-cell proliferation correlated with diminished intracellular Ca2+ signaling.
Conclusions:
- Sepsis-induced alterations in T-lymphocyte Ca2+ signaling negatively impact Con A-mediated proliferation.
- Impaired T-cell responsiveness during sepsis may contribute to reduced host resistance.