Related Experiment Video

Updated: Jul 19, 2026

Isolation of Rat Portal Fibroblasts by In situ Liver Perfusion
07:39

Isolation of Rat Portal Fibroblasts by In situ Liver Perfusion

Published on: June 29, 2012

Disposition of ciprofloxacin in the isolated perfused rat liver

A R Abadìa1, L De Francesco, A Guaitani

  • 1Experimental Liver Toxicology Unit, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.

Drug Metabolism and Disposition: the Biological Fate of Chemicals
|February 1, 1995
PubMed

More Related Videos

Isolation and Primary Culture of Rat Hepatic Cells07:06

Isolation and Primary Culture of Rat Hepatic Cells

Published on: June 29, 2012

Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control07:35

Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control

Published on: April 5, 2021

Related Concept Videos

Methods for Studying Drug Absorption: In situ01:09

Methods for Studying Drug Absorption: In situ

In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
Factors Affecting Drug Distribution: Organ Perfusion Rate01:15

Factors Affecting Drug Distribution: Organ Perfusion Rate

Drug distribution within the body is a complex process influenced by several factors, including perfusion rate, the rate at which the bloodstream transports drugs to tissue. This limitation becomes particularly significant when dealing with highly lipophilic drugs. In such cases, the rate at which the drug can move across membranes is crucial, and if the membrane is highly permeable to the drug, distribution becomes rate-limited by perfusion.
Perfusion rate-limited distribution relies on the...
Effect of Hepatic Disease on Pharmacokinetics: Drug Dosing and Hepatic Blood Flow01:26

Effect of Hepatic Disease on Pharmacokinetics: Drug Dosing and Hepatic Blood Flow

Chronic liver disease significantly impacts drug metabolism due to alterations in hepatic blood flow and enzyme accessibility. This disruption affects the body's pharmacokinetics—the movement and processing of drugs within the system. Key enzymes crucial for metabolizing medications become less accessible, changing how drugs are processed and utilized. Furthermore, liver disease influences the synthesis of plasma proteins, such as albumin and globulins, which play critical roles in drug binding...