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Use of conjugative and thermosensitive cloning vectors for transposon delivery to Mycobacterium smegmatis
J A Gavigan1, C Guilhot, B Gicquel
1Departamento de Microbiología, Facultad de Medicina, Universidad de Zaragoza, Spain.
FEMS Microbiology Letters
|March 15, 1995
Abstract:
Conjugative, thermosensitive shuttle plasmids capable of transfer from Escherichia coli to Mycobacterium smegmatis were constructed. They contain both an E. coli replicon and a thermosensitive derivative of the pAL5000 mycobacterial replicon. Using a temperature shift protocol, the conjugative plasmid, pJAZ11 was used to deliver the transposon Tn611 from E. coli into the chromosome of M. smegmatis. Analysis of transconjugants revealed the random insertion of the transposon.