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[Synthesis and characteristics of anti-CBG antibodies (anti-transcortin)]
We used corticosteroid binding globulin purified by chromatography on the Sepharose column for immunisation of the rabbits. We analysed serum by rocket immunoelectrophoresis and cross immunoelectrophoresis. The titre of the serum was estimated by reaction with 125 I-labelled transcortin (corticosteroid binding globulin). The results of our studies indicate that own serum is valuable and can be use for radioimmunoassay of the CBG in the serum.
We used corticosteroid binding globulin purified by chromatography on the Sepharose column for immunisation of the rabbits. We analysed serum by rocket immunoelectrophoresis and cross immunoelectrophoresis. The titre of the serum was estimated by reaction with 125 I-labelled transcortin (corticosteroid binding globulin). The results of our studies indicate that own serum is valuable and can be use for radioimmunoassay of the CBG in the serum.