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Lamin A precursor is localized to intranuclear foci
1Institut du cancer de Montréal, Centre de recherche Louis-Charles Simard, Hôpital Notre-Dame, Québec, Canada.
Journal of Cell Science
|January 1, 1995
Summary
The lamin A precursor can assemble into the nuclear lamina before isoprenylation. All processing steps to mature lamin A occur within the nucleus, even without isoprenylation.
Area of Science:
- Cell Biology
- Molecular Biology
- Protein Processing
Background:
- Lamin A undergoes complex post-translational modifications, including isoprenylation, proteolytic cleavage, and carboxymethylation.
- The precise subcellular localization of these processing steps for lamin A remains debated.
Purpose of the Study:
- To investigate the subcellular localization of lamin A precursor processing.
- To determine if isoprenylation is required for assembly into the nuclear lamina.
Main Methods:
- Production of a specific antibody against the lamin A precursor's carboxyl terminus.
- Immunofluorescence to detect precursor localization.
- Cell treatment with mevinolin (MVN) to inhibit isoprenoid synthesis and mevalonate (MVA) to restore it.
Main Results:
- The lamin A precursor, detected by the specific antibody, localizes to intranuclear foci.
- Mevinolin treatment caused accumulation of the precursor in the peripheral nuclear lamina.
- Addition of mevalonate led to the disappearance of the precursor from the lamina, restricting it to intranuclear foci.
Conclusions:
- The non-isoprenylated lamin A precursor can assemble into the peripheral nuclear lamina.
- All processing steps for mature lamin A can occur within the nuclear space.