Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Induction of delayed-type hypersensitivity with BCG-associated proteins

E D Crum, D D McGregor

    Immunology
    |April 1, 1976
    PubMed
    Summary

    Bovine serum albumin (BSA) and hen egg albumin (OA) bind to BCG, forming stable complexes. These complexes are potent inducers of delayed-type hypersensitivity (DTH), unlike soluble proteins alone.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Development of immunologically competent cells in the rat.

    Nature·2010
    Same author

    THE ANTIBODY RESPONSE OF RATS DEPLETED OF LYMPHOCYTES BY CHRONIC DRAINAGE FROM THE THORACIC DUCT.

    The Journal of experimental medicine·2009
    Same author

    Career paths of alumni of the Cornell Leadership Program for veterinary students.

    The Veterinary record·2008
    Same author

    Trichinella spiralis: the effect of specific antibody on muscle larvae in the small intestines of weaned rats.

    Experimental parasitology·1993
    Same author

    Effect of cisplatin upon expression of in vivo immune tumor resistance.

    Cancer immunology, immunotherapy : CII·1993
    Same author

    Intestinal mucus entrapment of Trichinella spiralis larvae induced by specific antibodies.

    Immunology·1991

    Area of Science:

    • Immunology
    • Microbiology
    • Biochemistry

    Background:

    • Proteins like bovine serum albumin (BSA) and hen egg albumin (OA) can interact with microorganisms.
    • The interaction between proteins and bacteria may influence immune responses.
    • Bacillus Calmette-Guérin (BCG) is an established immunomodulator.

    Purpose of the Study:

    • To investigate the spontaneous association of BSA and OA with living BCG.
    • To determine the stability of these protein-BCG complexes.
    • To evaluate the capacity of these complexes to induce delayed-type hypersensitivity (DTH).

    Main Methods:

    • Incubation of BCG with varying concentrations of BSA and OA in aqueous suspension.
    • Washing procedures to assess the stability of protein-BCG association.
    • Injection of protein-BCG complexes and soluble proteins (with or without BCG) into test subjects to assess DTH induction.

    Main Results:

    • Microgram quantities of BSA and OA spontaneously associate with living BCG, forming stable complexes resistant to dissociation.
    • Protein association is influenced by protein concentration, competing proteins, pH, and existing bound protein.
    • BCG complexes with BSA or OA are potent inducers of DTH, while soluble proteins are less effective, even when co-injected with BCG.

    Conclusions:

    • BCG serves as an effective carrier for BSA and OA, enhancing their immunogenicity.
    • The formation of stable protein-BCG complexes is crucial for potent DTH induction.
    • Antigen stabilization and concentration at the injection site by BCG contribute to cell-mediated immune responses.

    Related Experiment Videos