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Enhancement of feline infectious peritonitis virus type I infection in cell cultures using low-speed centrifugation
T Hohdatsu1, T Tatekawa, H Koyama
1Department of Veterinary Infectious Diseases, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
Abstract:
The effects of centrifugation on the ability of feline infectious peritonitis virus (FIPV) to infect cells in culture was investigated. The infectivity titer was the highest when the plates were centrifuged at 400 x g (1500 rpm) for 2 h. All five strains classified as FIPV Type I showed infectivity titers enhanced 10-100-fold by centrifugation at 400 x g for 2 h. The centrifugal enhancement of infection was obtained only by centrifugation immediately after inoculation of the virus, suggesting that the enhancement occurs during attachment or adsorption of viruses to the cells. This method may be useful for the culture of FIPV Type I strains.
Insights
Centrifugation significantly enhances feline infectious peritonitis virus (FIPV) infectivity in cell cultures. This method, particularly at 400 x g for 2 hours, improves FIPV Type I strain cultivation.
Area of Science:
- Veterinary Virology
- Cell Culture Techniques
- Infectious Disease Research
Background:
- Feline infectious peritonitis virus (FIPV) causes a significant disease in cats.
- Efficient cell culture methods are crucial for studying FIPV and developing treatments.
- Optimizing viral inoculation and culture conditions can improve experimental outcomes.
Purpose of the Study:
- To investigate the impact of centrifugation on FIPV infectivity in cell culture.
- To determine optimal centrifugation parameters for enhancing FIPV infection.
- To assess the utility of centrifugation for culturing FIPV Type I strains.
Main Methods:
- FIPV strains were cultured in cell monolayers.
- Cell cultures were centrifuged at various speeds (e.g., 400 x g) and durations (e.g., 2 hours) immediately after viral inoculation.
- Infectivity titers were measured to quantify viral replication and cell infection.
Main Results:
- Centrifugation at 400 x g for 2 hours resulted in the highest infectivity titers.
- All five tested FIPV Type I strains showed 10-100 fold enhancement in infectivity.
- Enhanced infection was observed only when centrifugation occurred immediately post-inoculation, suggesting an effect on viral attachment.
Conclusions:
- Centrifugation, specifically at 400 x g for 2 hours, significantly enhances FIPV Type I infectivity in cell culture.
- The enhancement is likely due to improved viral adsorption to host cells.
- This technique offers a valuable method for improving the culture and study of FIPV Type I strains.