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Incilaria mucus agglutinated human erythrocytes
E Furuta1, T Takagi, K Yamaguchi
1Department of Anatomy, Dokkyo University School of Medicine, Tochigi, Japan.
Abstract:
The body surface mucus from the land slug, Incilaria fruhstorferi, not only opsonized sheep red blood cells (SRBC) and agglutinated human types A and B erythrocytes (HRBC) but also caused hemolysis of B type HRBC 12 h after agglutination. The mucus-induced hemagglutination was specifically inhibited by a low concentration of N-acetyl D-galactosamine (GalNAc), and all of this activity was completely inactivated by heating at 56 degrees C for 30 min. SDS-polyacrylamide gel electrophoresis of water soluble fraction of the mucus showed that this fraction contained several proteins, the molecular mass of the main band being 35 KD.