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Structural plasticity of the nuclear pore complex

C W Akey1

  • 1Department of Biophysics, Boston University School of Medicine, Ma 02118-2394, USA.

Journal of Molecular Biology
|April 28, 1995
PubMed
Summary
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Structural plasticity of nuclear pore complexes (NPCs) was analyzed. Findings suggest NPC spoke architecture responds to nuclear envelope turgor pressure, influencing transport channel dimensions and transporter configurations.

Area of Science:

  • Cell Biology
  • Structural Biology
  • Biophysics

Background:

  • The nuclear pore complex (NPC) regulates transport between the nucleus and cytoplasm.
  • NPCs are located at the nuclear envelope, a double membrane structure.
  • Understanding NPC structural dynamics is crucial for comprehending nucleocytoplasmic transport.

Purpose of the Study:

  • To evaluate the structural plasticity of nuclear pore complexes (NPCs).
  • To investigate the relationship between NPC structure and nuclear envelope turgor pressure.
  • To characterize diffusion channels and transporter conformations within NPCs.

Main Methods:

  • Analysis of 4412 NPCs from isolated nuclear envelopes using correspondence analysis, classification, and difference mapping.
  • Comparison of detergent-extracted NPCs with published three-dimensional maps.

Related Experiment Videos

  • Generation of difference maps between membrane-associated and detergent-extracted NPCs.

    • Main Results:

    • NPCs were classified into seven clusters based on spoke radial compaction and inner spoke ring symmetry.
    • Symmetrical detergent-extracted NPCs differ from the most probable in vivo structure.
    • Proposed model suggests NPC spoke architecture is responsive to nuclear envelope turgor pressure, affecting spoke configuration and diffusion channel dimensions (0-20 Å x 190 Å).

    Conclusions:

    • NPC spoke deformations observed in isolated envelopes may be experimentally induced.
    • Concerted movements of spoke domains could be involved in NPC biological function.
    • Putative closed, open, and in-transit transporter forms were observed with equal frequency, irrespective of spoke conformation.