Application of the enzymic electric cell method to the activity assay of NAD-linked dehydrogenases

An enzymic electric cell was constructed with a saturated calomel electrode (cathode) and an enzymic electrode (anode) which consisted of a glassy carbon electrode and a mixture containing an NAD-linked dehydrogenase, NAD+, Nomethylphenazonium methosulfate and a substrate, and the short-circuit current of the cell was measured...