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The Schizosaccharomyces pombe MBF complex requires heterodimerization for entry into S phase
1Division of Neoplastic Disease Mechanisms, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
Molecular and Cellular Biology
|May 1, 1995
Summary
The MBF complex regulates gene expression for cell cycle progression. Its activity depends on the interaction between p85cdc10 and p72res1, crucial for the G1/S transition in Schizosaccharomyces pombe.
Area of Science:
- Molecular Biology
- Cell Cycle Regulation
- Yeast Genetics
Background:
- The MBF complex in Schizosaccharomyces pombe is implicated in activating genes for S phase entry.
- MBF comprises p85cdc10 and p72res1/sct1 subunits.
Purpose of the Study:
- To elucidate the regulatory role of p72res1 in MBF complex function.
- To understand how MBF controls gene expression during the G1/S transition.
Main Methods:
- Genetic analysis of p72res1.
- Gel mobility shift assays to assess DNA binding.
- In vivo overexpression studies of p72res1 fragments.
Main Results:
- p72res1 specifically binds the cdc22 promoter via its N-terminal 157 amino acids.
- Overexpression of the p72res1 DNA-binding domain rescues cdc10 mutants.
- The C-terminal region of p72res1 mediates p85cdc10 interaction, and its overexpression causes G1 arrest.
Conclusions:
- MBF activity is contingent upon the p85cdc10-p72res1 interaction.
- p72res1's DNA-binding and p85cdc10-binding domains have distinct roles in regulating MBF function and cell cycle progression.