Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

GAL4 interacts with TATA-binding protein and coactivators

K Melcher1, S A Johnston

  • 1Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235-8573, USA.

Molecular and Cellular Biology
|May 1, 1995
PubMed
Summary

The GAL4 activation domain directly binds TATA-binding protein (TBP), a key transcription factor. This interaction is crucial for gene regulation and suggests similar binding mechanisms for different transcriptional activators.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Zebrafish Larvae as an Experimental Model of Cryptococcal Meningitis.

Methods in molecular biology (Clifton, N.J.)·2023
Same author

Technology of Informative Feature Selection for Immunosignature Analysis.

Sovremennye tekhnologii v meditsine·2021
Same author

First Report of Powdery Mildew in Greenhouse-Grown Tomatoes in New Jersey.

Plant disease·2019
Same author

An Assessment of Fungicide Benefits for the Control of Fungal Diseases of Processing Tomatoes in New York and New Jersey.

Plant disease·2019
Same author

Experiences of rosacea and its treatment: an interpretative phenomenological analysis.

The British journal of dermatology·2017
Same author

The evaluation of analgesia provided by epidural ketamine in dogs with chemically induced synovitis.

Veterinary anaesthesia and analgesia·2017

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Biochemistry

Background:

  • Identifying transcriptional activator targets is crucial for understanding eukaryotic gene regulation.
  • Previous research has suggested various candidate targets for transcriptional activators.

Purpose of the Study:

  • To identify the specific protein targets of the GAL4 activation domain (AD).
  • To characterize the interaction between the GAL4 AD and its identified targets, particularly TATA-binding protein (TBP).

Main Methods:

  • Protein retention assays using a GAL4 carboxy-terminal peptide as bait.
  • In vitro binding assays to assess the specificity, directness, and salt stability of the GAL4 AD-TBP interaction.
  • Analysis of yeast TBP mutations to evaluate their impact on GAL4 AD binding affinity.

Related Experiment Videos

Main Results:

  • The GAL4 AD retained GAL80, TBP, SUG1, and ADA2 from a crude extract.
  • The interaction between the GAL4 AD and TBP was shown to be specific, direct, and salt-stable.
  • Mutations in the GAL4 AD affecting in vivo transcriptional activation also affected TBP binding affinity.
  • A specific mutation in yeast TBP similarly reduced binding to both GAL4 and VP16 activation domains.

Conclusions:

  • The GAL4 activation domain directly interacts with TATA-binding protein (TBP).
  • This direct interaction is essential for transcriptional activation.
  • The findings suggest that different transcriptional activators may utilize similar contact points on TBP.