A simple technique for observing germ tube formation in Candida albicans

Candida albicans is identified in most laboratories by the rapid formation of germ tubes when it is grown in human serum (Taschdjian et al, 1960). Demonstration of the production of germ tubes on solid media, 1% bactopeptone in 2% agar (Joshi and Gavin, 1974), and the appearance of other yeasts on a variety of carbohydrate media (Joshi et al, 1975) has led to the development of a simple technique for observing germ tube production.