Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-performance capillary electrophoresis in the pharmaceutical sciences

L J Brunner1, J T DiPiro, S Feldman

  • 1Department of Pharmaceutics, College of Pharmacy, University of Georgia, Athens, USA.

Pharmacotherapy
|January 1, 1995
PubMed
Summary

High-performance capillary electrophoresis (HPCE) offers rapid analysis and automation in pharmaceutical sciences. While it has a high detection limit, HPCE provides a valuable new dimension for laboratory separation techniques.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy of a single intravenous dose of amphotericin B for Candida urinary tract infections: further favorable experience.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2003
Same author

Impact of clinical pharmacy services on renal transplant patients' compliance with immunosuppressive medications.

Clinical transplantation·2001
Same author

In vivo induction of hepatic p-glycoprotein by cyclosporine in the rat.

Research communications in molecular pathology and pharmacology·2001
Same author

Medication assistance programs.

Annals of internal medicine·2001
Same author

Effect of low dose cyclosporine and sirolimus on hepatic drug metabolism in the rat1.

Transplantation·2001
Same author

Chronic cyclosporine administration induces renal P-glycoprotein in rats.

European journal of pharmacology·2001

Area of Science:

  • Pharmaceutical Sciences
  • Analytical Chemistry

Background:

  • High-performance capillary electrophoresis (HPCE) is an emerging separation technique.
  • HPCE utilizes narrow capillaries and electrical fields for sample component separation.

Purpose of the Study:

  • To introduce and describe the principles and applications of HPCE in pharmaceutical sciences.
  • To highlight the advantages and disadvantages of HPCE compared to traditional methods.

Main Methods:

  • Separations are performed in narrow-bore capillaries under an applied electrical field.
  • Component migration is based on physicochemical properties like charge, mass ratio, and lipid solubility.
  • On-line detection is achieved through a capillary detection window.

Main Results:

Related Experiment Videos

  • HPCE enables rapid analysis times, automation, and requires minimal sample volumes.
  • A significant limitation of HPCE is its high concentration limit of detection.
  • The technique separates components based on properties such as ionic charge and lipid solubility.

Conclusions:

  • HPCE is a valuable addition to existing laboratory separation techniques.
  • It will complement, not replace, traditional separation methods in pharmaceutical analysis.
  • The technique offers unique advantages for specific analytical challenges.