[Immunoenzyme analysis based on laccase conjugates with fluorometric detection of the enzymatic reaction product]

The possibility of using homovanillic acid as a substrate of laccase (produced by the basidiomycete Coryolus hirsutus) has been demonstrated for the first time. The reaction was shown to result in the formation of a fluorescent product. Several kinetic parameters and optimal conditions were determined for this enzymatic reaction. The use of homovanillic acid as the substrate was found to increase the enzyme immunoassay sensitivity by an order of magnitude, compared to conventional substrates.