[Can parameters of amperometric single-enzyme sensors be assessed using the concentration dependence of their...

The mathematical models of amperometric biosensors, with single- or two-substrate reaction occurring in their cover, were developed. A linear two-parametric equation of steady state was suggested for the biosensor detecting the product of single-substrate reaction corresponding to the Michaelis-Menten scheme. The conditions of equation applicability to description of standard biosensor kinetics in the wide range of substrate concentration, diffusion-module values, and permeability coefficients of three-layer-cover components were studied.