[Replacement of native sera with growth-stimulating proteins during culturing of mammalian cells]

The authors recommend to replace nonstandard native animal sera by growth-stimulating proteins isolated from the sera of cattle, northern deer, pigs, and sheep by fractionation with polyethylene glycol aqueous solutions (mol. m 6000 dalton). Growth-stimulating proteins are conducive to active proliferation of primary and continuous cell cultures at various methods of culturing. Growth-stimulating proteins were for the first time added to nutrient media during culturing of mammalian cells on microcarriers.