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Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
Published on: May 1, 2014
In vitro effects of retinoic acid on mouse incisor development
A Bloch-Zupan1, M P Mark, B Weber
1Institut de Biologie Médicale, INSERM-Université Louis Pasteur, Faculté de Médecine, Strasbourg, France.
Archives of Oral Biology
|October 1, 1994
Summary
Retinoic acid (RA) is crucial for mouse incisor development, controlling initial tooth shape and cell division. Its effects are independent of cytoplasmic RA-binding proteins (CRABPs).
Area of Science:
- Developmental Biology
- Oral Biology
- Molecular Biology
Background:
- The developing dentition expresses retinoic acid (RA) receptors and binding proteins.
- RA is essential for in vitro mouse molar development.
Purpose of the Study:
- To investigate the role of RA in in vitro mouse incisor development.
- To understand RA's influence on incisor morphogenesis and cellular activity.
Main Methods:
- Tooth germ cultures were analyzed with and without RA.
- Histology, immunocytochemistry, immunofluorescence, and in situ hybridization were employed.
- Proliferating cells, laminin deposition, and CRABP gene transcripts were examined.
Main Results:
- RA controlled initial incisor morphogenesis, including cervical loop asymmetry and mitotic activity.
- RA influenced asymmetrical laminin deposition.
- CRABPI gene expression in the labial epithelium required RA in vitro, but RA's effects were independent of CRABP binding.
Conclusions:
- RA plays a significant role in regulating mouse incisor development.
- RA controls key morphogenetic events and cellular proliferation.
- The observed effects of RA are not mediated through direct binding to CRABPs.
