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Hydration and order in lipid bilayers

C Ho1, S J Slater, C D Stubbs

  • 1Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.

Biochemistry
|May 9, 1995
PubMed
Summary
This summary is machine-generated.

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Membrane lipid bilayer hydration and acyl chain order are linked, especially in the interchain region. This study reveals a strong correlation between interchain hydration and acyl chain order, regardless of lipid composition.

Area of Science:

  • Biophysics
  • Membrane Biophysics
  • Lipid Bilayer Dynamics

Background:

  • Membrane lipid bilayer hydration is crucial for membrane function.
  • Acyl chain order influences membrane fluidity and permeability.
  • Understanding the interplay between hydration and order is key to elucidating membrane properties.

Purpose of the Study:

  • To investigate the relationship between membrane lipid bilayer hydration and acyl chain order.
  • To differentiate hydration effects in the head group versus acyl chain regions.
  • To explore how lipid unsaturation and cholesterol affect these properties.

Main Methods:

  • Time-resolved fluorescence spectroscopy using dansyl-PE and DPH-PC probes.
  • Fluorescence lifetime and intensity measurements in H2O and D2O buffers.

Related Experiment Videos

  • Time-resolved anisotropy measurements to determine acyl chain order.

    • Main Results:

    • Head group hydration was assessed using dansyl-PE, while interchain hydration and acyl chain order were evaluated using DPH-PC.
    • Lipid unsaturation showed distinct effects on interchain and head group hydration.
    • Cholesterol exhibited opposing effects on interchain and head group hydration.
    • A strong correlation was found between interchain hydration and acyl chain order, independent of lipid type.

    Conclusions:

    • Interchain hydration is strongly correlated with acyl chain order in lipid bilayers.
    • This correlation is independent of specific lipid composition, suggesting a fundamental biophysical principle.
    • Distinct hydration dynamics exist in the head group and acyl chain regions of the membrane.