Related Experiment Videos
A role of sigma receptors on hypoxia/hypoglycemia-induced decrease in CA1 presynaptic fiber spikes in rat hippocampal
S Shibata1, Y Yamamoto, S Watanabe
1Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyushu University 62, Fukuoka, Japan.
Brain Research
|January 30, 1995
Summary
Sigma receptor antagonists protect against hypoxia/hypoglycemia-induced neuronal damage in rat hippocampal slices. Sigma receptor stimulation appears to worsen these neurophysiological impairments.
Area of Science:
- Neuroscience
- Pharmacology
- Cellular Biology
Background:
- Hypoxia and hypoglycemia can cause significant neuronal damage, particularly in vulnerable brain regions like the hippocampus.
- Sigma receptors are implicated in various neurological processes, but their role in neuroprotection under ischemic conditions is not fully understood.
Purpose of the Study:
- To investigate the effects of sigma receptor agonists and antagonists on neuronal function in rat hippocampal slices under conditions of hypoxia and hypoglycemia.
- To elucidate the role of sigma receptors in mediating neurophysiological changes during oxygen and glucose deprivation.
Main Methods:
- Experiments were conducted using rat hippocampal slices.
- Schaffer collaterals were stimulated to elicit CA1 presynaptic fiber spikes.
- The effects of various sigma receptor agonists and antagonists (haloperidol, NE-100, rimcazole, DTG, SKF10047) on hypoxia/hypoglycemia-induced decreases in CA1 presynaptic potential were assessed.
Main Results:
- Sigma receptor antagonists (haloperidol, NE-100, rimcazole) demonstrated a concentration-dependent protective effect against hypoxia/hypoglycemia-induced decreases in CA1 presynaptic fiber spikes.
- The potency order for antagonists was NE-100 = haloperidol > rimcazole.
- The sigma receptor agonist DTG exacerbated the neurophysiological deficit, while SKF10047 showed a protective effect.
- NE-100 counteracted the detrimental effects of DTG but did not affect the protective action of SKF10047.
Conclusions:
- Sigma receptor stimulation appears to facilitate hypoxia/hypoglycemia-induced impairment of neurophysiological functions in the CA1 presynaptic region of hippocampal slices.
- These findings suggest that modulating sigma receptor activity could be a potential therapeutic strategy for conditions involving ischemic brain injury.