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A novel immunosuppressive factor in human colostrum

P Mandalapu1, H F Pabst, V Paetkau

  • 1Department of Biochemistry, University of Alberta, Edmonton, Canada.

Cellular Immunology
|May 1, 1995
PubMed
Summary
This summary is machine-generated.

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Human colostrum contains a colostrum inhibitory factor (CIF) that blocks interleukin-2 (IL2) production in T cells. This factor, found in high concentrations and enhanced by acidification, is distinct from TGF-beta.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Human colostrum possesses a unique inhibitory factor affecting T lymphocyte function.
  • Interleukin-2 (IL2) induction is critical for T cell activation and immune response.

Purpose of the Study:

  • To characterize the colostrum inhibitory factor (CIF) responsible for inhibiting IL2 induction.
  • To investigate the properties and distinctiveness of CIF compared to known growth factors like TGF-beta.

Main Methods:

  • Assessing IL2 mRNA accumulation in PMA-stimulated EL4.E1 T lymphocyte cell lines.
  • Quantifying CIF activity in human colostrum and its response to pH changes.
  • Comparing CIF's inhibitory effects with those of TGF-beta using specific antisera and cell stimulation protocols.

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Main Results:

  • Human colostrum contains approximately 100 U/ml of CIF activity, inhibiting IL2 production by blocking IL2 mRNA accumulation.
  • Acidification to pH 3.0 significantly enhances CIF activity.
  • CIF activity is largely independent of TGF-beta, as TGF-beta antibodies neutralized less than 10% of CIF activity, and CIF demonstrated more potent inhibition than TGF-beta.

Conclusions:

  • A novel colostrum inhibitory factor (CIF) effectively blocks IL2 induction in T cells.
  • CIF exhibits distinct biochemical properties and a stronger inhibitory effect compared to TGF-beta.
  • The presence of CIF in bovine colostrum suggests a conserved biological role in milk-derived immune modulation.