Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Microglia activation after neonatal hypoxic-ischemia

A McRae¹, E Gilland, E Bona

¹Department of Anatomy and Cell Biology, University of Göteborg, Sweden.

Brain Research. Developmental Brain Research

|February 16, 1995

Summary

Neonatal hypoxic-ischemia (HI) triggers an inflammatory response involving microglial activation. This response varies in timing across brain regions, impacting neuronal injury markers like microtubule associated protein II (MAP II).

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Uterus transplantation; first data on neurologic, neuropsychiatric, and physical examination follow-up of children up to 6 years of age.

Human reproduction (Oxford, England)·2025

Same author

Tissue informative cell-free DNA methylation sites in amyotrophic lateral sclerosis.

medRxiv : the preprint server for health sciences·2024

Same author

Cost-effectiveness of cervical length screening and progesterone treatment to prevent spontaneous preterm delivery in Sweden.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2022

Same author

Benefit of pazopanib in advanced gastrointestinal stromal tumours: results from a phase II trial (SSG XXI, PAGIST).

ESMO open·2021

Same author

Second-trimester transvaginal ultrasound measurement of cervical length for prediction of preterm birth: a blinded prospective multicentre diagnostic accuracy study.

BJOG : an international journal of obstetrics and gynaecology·2020

Same author

Inositols and metabolic disorders: From farm to bedside.

Journal of traditional and complementary medicine·2020

Area of Science:

Neuroscience
Immunology
Neonatal Research

Background:

The inflammatory response after neonatal hypoxic-ischemia (HI) is not well understood.
Microglial activation and beta-amyloid precursor protein (APP) expression are key areas of interest.

Purpose of the Study:

To investigate microglial antigen expression and APP in relation to neuronal injury markers after neonatal HI.
To characterize the temporal and regional patterns of microglial response and neuronal damage.

Main Methods:

Neonatal rats (7-day-old) underwent unilateral carotid ligation and hypoxia to induce HI.
Immunohistochemistry was used to detect microglial antigens (OX-18, OX-6, OX-42), APP, and MAP II at various time points post-HI.

Main Results:

Related Experiment Videos

Transient APP expression was observed 2-3 hours after HI.
Microglial activation (OX-18, OX-6, OX-42) showed a biphasic pattern: acute in some areas (24h-4 days) and delayed in others (thalamus, 14 days).
Delayed microglial response in the thalamus correlated with delayed loss of MAP II, a neuronal injury marker.

Conclusions:

Neonatal HI induces significant microglial activation with distinct temporal profiles depending on the brain region.
The timing of microglial response influences the extent and timing of neuronal injury, as indicated by MAP II loss.