[Distribution of ABH substances in normal secretor human tissue cells by avidin-biotin complex method]

The distribution and location of ABH substances in 54 various kinds of normal secretor human tissue cell of known ABO type were studied by Avidin-Biotin complex (ABC) method. It was firstly demonstrated that 18 different kinds of tissue cells contained ABH substances, which were as follows: neuron and nerve fiber, astrocyte, oligodendrocyte, ependyma epithelial cells, epithelial cells of soft meninges, epithelial cells of parotid gland duct, taste bud cells of tongue, acinar and epithelial cells of duct of buccal gland, epithelial cells of rectal mucous membrane gland, regenerated liver cells of hepatocirrhosis, basal cells of stratified aquamous epithelium of epiglottis, spermiogenesis and spermatozoa of seminiferous tubules, epithelial cells of tubuli reti, rete testis and tubuli epididymis, both primary and secondary follicles of ovary and decidua cells of endometrium. Some new phenomena were observed as follows: ABH substances in chief cells more than those in parietal cells of gastric gland, locally distributed ABH positive cells in stratum spinosum of stratified squamous epithelium of skin, segmentally distributed ABH positive cells in pseudostratified ciliated columanal epithelium of epiglottis. The ABH substances were located at the infranuclear and/or supranuclear region and/or brush border of the epithelial cells of submaxillary gland, mosaic distributed ABH substances were found in the epithelia of both sweat and submaxillary glandular ducts. A few controversies about distribution of ABH substances were solved. The relationship between H and A, B substances, origin of ABH substances, source of ABH substances in body fluids. Production of ABH substances and cell differentiation, mosaicism and inconsistency of ABH distribution are discussed and compared with other reports.(ABSTRACT TRUNCATED AT 250 WORDS)