Cryo-immunogold ultrastructural localization of laminin in adult rat peripheral nerve

Elucidation of the functional roles of the extracellular matrix component laminin in adult peripheral nerve has been hindered by differing accounts of its ultrastructural localization. This is the first report applying the advantages of the cryo-immunogold technique to laminin localization in peripheral nerve. Laminin labelling was found over the basal lamina and possibly over the immediately subjacent Schwann cell plasma membrane, but specific labelling appeared to be absent from other membranes (including those of non-myelinated axon/Schwann cell clusters) and from endoneurial collagen fibrils. It would appear that the functional roles played by laminin in normal adult peripheral nerve are likely to be mediated via its localization in the basal lamina, rather than through a more widespread distribution within the endoneurium.