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Changes in lymphocyte beta 2-adrenoceptors after hepatic resection
H Terajima1, K Mori, T Taniguchi
1Second Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
The Journal of Surgical Research
|May 1, 1995
Summary
Hepatic resection significantly reduces lymphocyte beta 2-adrenoceptor binding capacity due to surgical stress. A lower arterial ketone body ratio indicates less stress and preserves receptor function.
Area of Science:
- Immunology
- Surgical Stress Response
- Cellular Signaling
Background:
- Hepatic resection induces significant surgical stress, potentially impairing vital functions and signal transduction.
- Lymphocyte beta 2-adrenoceptors are crucial in immune responses and may be affected by surgical stress.
Purpose of the Study:
- To investigate the impact of surgical stress from hepatic resection on ligand-receptor binding activity.
- To specifically examine changes in lymphocyte beta 2-adrenoceptor binding parameters post-hepatectomy.
Main Methods:
- Radioligand binding assay using (-)3H-CGP12177 to determine maximum binding capacity (Bmax) and dissociation constant (KD).
- Comparison of receptor binding parameters in hepatectomy patients versus a control group.
- Analysis of receptor changes based on postoperative arterial ketone body ratio (AKBR).
Main Results:
- Hepatectomy significantly decreased Bmax of lymphocyte beta 2-adrenoceptors on postoperative days 3 and 7 (P < 0.05).
- No significant changes in KD were observed in either group.
- A lower AKBR (Group B) was associated with a smaller decrease in Bmax compared to a higher AKBR (Group A), suggesting less stress.
Conclusions:
- Intense surgical stress from hepatic resection negatively affects ligand-receptor binding parameters.
- The decrease in AKBR can serve as an indicator for the magnitude of surgical stress experienced by patients.