Jove
Visualize
Contact Us

Related Experiment Videos

Structural alterations in Candida albicans by caffeine and caffeine salts

H Mittag1

  • 1Medizinisches Zentrum für Hautkrankheiten, Philipps-Universität Marburg, Germany.

Mycoses
|September 1, 1994
PubMed
Summary

Subinhibitory caffeine concentrations altered Candida albicans proliferation, causing multiple budding. These yeast cells structurally resembled Paracoccidioides brasiliensis, differing from ketoconazole effects.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diffuse vegetating bromoderma.

Journal of the European Academy of Dermatology and Venereology : JEADV·2019
Same author

Three different autoimmune bullous diseases in one family: is there a common genetic base?

The British journal of dermatology·1999
Same author

Desquamative gingivitis and balanitis--linear IgA disease or cicatricial pemphigoid?

Clinical and experimental dermatology·1999
Same author

[Morphology of Malassezia-associated diseases].

Mycoses·1996
Same author

Palmar papillomatous lesions reminiscent of epidermal nevus in a case of focal dermal hypoplasia: a nosological consideration.

Dermatology (Basel, Switzerland)·1996
Same author

Fine structural investigation of Malassezia furfur. II. The envelope of the yeast cells.

Mycoses·1995
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Mycology
  • Microbiology
  • Cell Biology

Background:

  • Candida albicans is an opportunistic fungal pathogen.
  • Understanding its response to various compounds is crucial for antifungal development.
  • Caffeine's biological effects are widely studied, but its impact on C. albicans cytology is less explored.

Purpose of the Study:

  • To investigate the cytological effects of subinhibitory caffeine and caffeine salts on Candida albicans.
  • To compare caffeine's effects with a known antifungal, ketoconazole.
  • To characterize the morphological changes induced by caffeine exposure.

Main Methods:

  • Light microscopy and transmission electron microscopy (TEM) were employed.
  • Candida albicans cultures were exposed to subinhibitory concentrations of caffeine and caffeine salts.

Related Experiment Videos

  • Comparative analysis with ketoconazole treatment was performed.

    • Main Results:

    • Caffeine and caffeine salts induced unusual proliferation modes in Candida albicans.
    • Multiple budding was observed as a significant morphological alteration.
    • The observed cytological changes differed from those induced by ketoconazole.
    • Caffeine-treated yeast cells showed structural similarities to Paracoccidioides brasiliensis.

    Conclusions:

    • Subinhibitory caffeine concentrations induce distinct cytological alterations in Candida albicans.
    • Caffeine promotes atypical budding, suggesting a unique mechanism of action.
    • The morphological resemblance to Paracoccidioides brasiliensis warrants further investigation.