Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bacterial protein kinases that recognize tertiary rather than primary structure?

M H Saier1

  • 1Department of Biology, University of California, San Diego, La Jolla 92093-0116, USA.

Research in Microbiology
|November 1, 1994
PubMed
Summary

Bacterial protein kinases recognize protein tertiary structure, unlike eukaryotic kinases that use primary structure. This suggests independent evolution and distinct mechanisms for these bacterial enzymes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mathematics: The Basis for Quantitative Knowledge.

Water, air, and soil pollution·2012
Same author

A Vaccine Against Ignorance?

Water, air, and soil pollution·2011
Same author

The Legacy of Oil Spills.

Water, air, and soil pollution·2010
Same author

Survival of the Fattest.

Water, air, and soil pollution·2010
Same author

Beneficial bacteria and bioremediation.

Journal of molecular microbiology and biotechnology·2005
Same author

Evolution of the bacterial phosphotransferase system: from carriers and enzymes to group translocators.

Biochemical Society transactions·2005

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Eukaryotic protein kinases typically recognize primary amino acid sequence for substrate phosphorylation.
  • Recent findings indicate some bacterial protein kinases phosphorylate targets based on tertiary structure.

Purpose of the Study:

  • To investigate the distinct mechanisms of bacterial protein kinases.
  • To propose an evolutionary divergence in protein kinase function.

Main Methods:

  • Comparative analysis of eukaryotic and bacterial protein kinase substrate recognition.
  • Bioinformatic analysis of protein kinase families.
  • Structural biology studies (implied).

Main Results:

Related Experiment Videos

  • Eukaryotic protein kinases recognize primary protein structure.
  • Certain bacterial protein kinases recognize tertiary protein structure.
  • Evidence suggests independent evolutionary origins for these kinase types.

    • Conclusions:

    • Bacterial protein kinases utilizing tertiary structure recognition likely evolved independently from eukaryotic kinases.
    • The modes of target recognition and action differ significantly between these bacterial and eukaryotic enzymes.