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Related Experiment Videos

Reverse transcription PCR to detect enteroviruses in surface water

M Gilgen1, B Wegmüller, P Burkhalter

  • 1Laboratory of Food Chemistry, University of Bern, Switzerland.

Applied and Environmental Microbiology
|April 1, 1995
PubMed
Summary

A new, rapid method effectively detects enteroviruses in water. This technique is suitable for routine water quality monitoring to identify viral contamination and ensure public health safety.

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Area of Science:

  • Environmental Microbiology
  • Virology
  • Molecular Biology

Background:

  • Enteroviruses pose a significant public health risk when present in water sources.
  • Accurate and efficient detection methods are crucial for monitoring water quality and preventing disease outbreaks.
  • Existing methods for enterovirus detection can be time-consuming and complex.

Purpose of the Study:

  • To develop a simple, fast, and efficient procedure for detecting enteroviruses in water samples.
  • To establish a reliable method for routine water quality assessment regarding viral contamination.
  • To compare the presence of enteroviruses with bacterial contamination indicators.

Main Methods:

  • A two-step filtration process using a positively charged nylon membrane to capture virus particles.

Related Experiment Videos

  • Direct lysis of adsorbed viruses and RNA isolation using magnetic beads and specific oligonucleotides.
  • Detection and identification via reverse transcription PCR (RT-PCR) of conserved viral RNA regions, followed by Southern hybridization.
  • Testing with spiked tap water and natural surface water samples.

    • Main Results:

    • The developed procedure achieved a detection limit of 20-50% tissue culture infective doses per liter in spiked tap water.
    • Out of 40 surface water samples tested, 7 were found to be positive for enteroviruses.
    • Enteroviruses were detected only in samples that also showed contamination with Escherichia coli.

    Conclusions:

    • The developed procedure is simple, fast, and efficient for detecting enteroviruses in water.
    • This method is suitable for routine water quality monitoring and assessing virus contamination.
    • The findings highlight the importance of monitoring both viral and bacterial indicators for comprehensive water safety assessment.