Related Experiment Video

Updated: Oct 1, 2026

A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry
08:26

A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry

Published on: April 9, 2013

The functional role of 55- and 75-kDa tumour necrosis factor receptors in human polymorphonuclear cells in vitro

Y Abe1, Y Osuka, T Nakata

  • 1Second Department of Surgery, Ehime University School of Medicine, Japan.

Cytokine
|January 1, 1995
PubMed

Insights

Human polymorphonuclear cells (PMN) primarily express tumour necrosis factor-receptor II (TNF-RII), but signalling of lymphotoxin (LT) and TNF primarily occurs via TNF-RI. This study investigates their distinct roles in PMN function.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Tumour necrosis factor-receptors (TNF-R) mediate crucial cellular responses.
  • Polymorphonuclear cells (PMN) play a key role in inflammatory processes.
  • Understanding TNF-R function in PMN is vital for inflammatory disease research.

Purpose of the Study:

  • To elucidate the distinct roles of TNF-RI and TNF-RII in human PMN.
  • To investigate the signaling pathways of lymphotoxin (LT) and TNF in PMN.
  • To determine the impact of TNF-R activation on PMN functions like superoxide release, granule exocytosis, and adhesion.

Main Methods:

  • Utilized agonistic rabbit polyclonal anti-TNF-R antibodies to stimulate TNF-RI and TNF-RII.
  • Studied human PMN in vitro.
  • Assessed superoxide anion release, elastase and lactoferrin release, and Mac-1 expression.

Main Results:

  • PMN predominantly express TNF-RII, but LT/TNF signaling is mainly mediated by TNF-RI.
  • Anti-TNF-RI antibody stimulated superoxide release, elastase, and lactoferrin release, mimicking LT.
  • Anti-TNF-RI antibody upregulated PMN adhesiveness and Mac-1 expression; TNF-RII modulated adhesion when TNF-RI was stimulated.

Conclusions:

  • Human PMN primarily express TNF-RII, but TNF-RI mediates key LT/TNF signaling.
  • TNF-RI is the primary mediator of LT-induced superoxide release, granule exocytosis, and adhesion molecule upregulation in PMN.
  • TNF-RII plays a modulatory role in PMN adhesion, particularly in conjunction with TNF-RI activation.

More Related Videos

Isolation and Characterization of Neutrophils with Anti-Tumor Properties10:15

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Published on: June 19, 2015

Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody (mAb) by Neutralizing TNF Using an In Vitro Bioanalytical Method16:07

Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody (mAb) by Neutralizing TNF Using an In Vitro Bioanalytical Method

Published on: September 16, 2017

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...