Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Inflammation-induced decrease in hepatic cytochrome P450 in conscious rabbits is accompanied by an increase in

M Proulx1, P du Souich

  • 1Département de Pharmacologie, Faculté de Médecine Université de Montréal, Québec, Canada.

Research Communications in Molecular Pathology and Pharmacology
|February 1, 1995
PubMed
Summary

A subcutaneous inflammatory reaction in rabbits induced reactive oxygen species (ROS) formation in the liver. This inflammation reduced cytochrome P450 and increased oxidative stress, impacting liver function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Of logos and men: semantic memory impairment for unique entities in a case of semantic variant of primary progressive aphasia.

Neurocase·2020
Same author

Anthropometric measures and their association with risk factors for cardio-metabolic diseases in Kenyan adults.

Annals of human biology·2019
Same author

Comments on "OARSI guidelines for the non-surgical management of knee osteoarthritis".

Osteoarthritis and cartilage·2014
Same author

A multicentre open-label safety and efficacy study of tetrodotoxin for cancer pain.

Current oncology (Toronto, Ont.)·2011
Same author

Does predation risk influence habitat use by northern redbelly dace Phoxinus eos at different spatial scales?

Journal of fish biology·2010
Same author

Modulation of inflammation by chondroitin sulfate.

Osteoarthritis and cartilage·2010

Area of Science:

  • Biochemistry
  • Toxicology
  • Pathology

Background:

  • Cytokines and local inflammation are known to increase reactive oxygen species (ROS) in the liver.
  • The effect of a systemic subcutaneous (s.c.) inflammatory reaction on hepatic ROS production in vivo remains unclear.
  • Previous research suggests inflammation may decrease hepatic cytochrome P450 levels.

Purpose of the Study:

  • To investigate whether an in vivo subcutaneous inflammatory reaction induces ROS formation in the liver.
  • To determine if this inflammation affects hepatic cytochrome P450 levels and activity.
  • To assess the impact on oxidative stress markers in the liver.

Main Methods:

  • Subcutaneous injection of turpentine or saline in rabbits.
  • Assessment of hepatic cytochrome P450 amount and activity (aniline hydroxylation, aminopyrine demethylation).

Related Experiment Videos

  • Measurement of lipid peroxidation, enzymatic scavenger activity (catalase, glutathione peroxidase, superoxide dismutase), reduced glutathione, and xanthine oxidase system activity.

    • Main Results:

    • Subcutaneous inflammation significantly reduced total hepatic cytochrome P450 and its activity.
    • Increased lipid peroxidation and decreased activity of antioxidant enzymes (catalase, glutathione peroxidase, superoxide dismutase) were observed.
    • Hepatic reduced glutathione levels diminished, while xanthine oxidase system activity increased by nearly 200%, indicating enhanced ROS production.

    Conclusions:

    • In vivo subcutaneous inflammation induces oxidative stress in the liver.
    • The inflammatory reaction leads to a decrease in hepatic cytochrome P450 and its activity.
    • These findings suggest a direct link between systemic inflammation and hepatic oxidative damage.