Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Augmented urokinase receptor expression in atheroma

H Noda-Heiny1, A Daugherty, B E Sobel

  • 1Cardiovascular Division, Washington University School of Medicine, St Louis, MO 63110, USA.

Arteriosclerosis, Thrombosis, and Vascular Biology
|January 1, 1995
PubMed
Summary

Increased expression of urokinase plasminogen activator receptor (uPA-R) on vascular smooth muscle cells in atheroma promotes cell migration and neointimalization, contributing to atherosclerosis development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Disruption of the cathepsin K gene reduces atherosclerosis progression and induces plaque fibrosis but accelerates macrophage foam cell formation.

Circulation·2005
Same author

Paradoxical reduction of atherosclerosis in apoE-deficient mice with obesity-related type 2 diabetes.

Cardiovascular research·2003
Same author

Antagonism of AT2 receptors augments angiotensin II-induced abdominal aortic aneurysms and atherosclerosis.

British journal of pharmacology·2001
Same author

Atherosclerosis: cell biology and lipoproteins.

Current opinion in lipidology·2001
Same author

Freunds adjuvant alone is antiatherogenic in apoE-deficient mice and specific immunization against TNFalpha confers no additional benefit.

Atherosclerosis·2001
Same author

Macrophage-specific expression of class A scavenger receptors enhances granuloma formation in the absence of increased lipid deposition.

Journal of lipid research·2001

Area of Science:

  • Cardiovascular Biology
  • Cell Biology
  • Atherosclerosis Research

Background:

  • Smooth muscle cell proliferation and migration are key processes in atherosclerosis.
  • The role of plasminogen activators and their receptors in atherogenesis is not fully understood.
  • The receptor for urokinase-type plasminogen activator (uPA-R) is a potential mediator of these cellular events.

Purpose of the Study:

  • To investigate the expression and localization of uPA-R in atherosclerotic lesions.
  • To determine the role of uPA-R in vascular smooth muscle cell migration in atheroma.

Main Methods:

  • Immunostaining of atherosclerotic lesions from rabbits and humans to detect uPA-R.
  • Western blotting to quantify uPA-R protein levels in normal and diseased vessels.

Related Experiment Videos

  • In vitro studies using cultured vascular smooth muscle cells and antibodies to uPA-R.

    • Main Results:

    • uPA-R was highly expressed in the neointima of atherosclerotic lesions but not in normal arteries.
    • uPA-R was localized to macrophages and smooth muscle cells within the neointima.
    • uPA-R protein levels were significantly higher in atheroma compared to normal vessels.
    • Antibodies targeting uPA-R inhibited vascular smooth muscle cell migration in vitro.
    • uPA-R mRNA levels did not significantly increase in migrating cells, suggesting posttranscriptional regulation.

    Conclusions:

    • Increased cell-surface uPA-R in atheroma contributes to pericellular proteolysis.
    • Elevated uPA-R promotes vascular smooth muscle cell migration, leading to neointimalization in atherosclerosis.
    • Altered posttranscriptional regulation may be responsible for increased uPA-R levels in atheroma.