Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hormone effects on hepatic substrate preference in sepsis

C N Paidas1, M G Clemens

  • 1Division of Pediatric Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA.

Shock (Augusta, Ga.)
|February 1, 1994
PubMed
Summary

Septic rat livers show altered substrate handling. Catecholamine stimulation impacts gluconeogenesis and ureagenesis differently in sepsis, affecting nutrient metabolism.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Time-dependent Effects of Pressure during Preservation of Rat Hearts in an Isochoric System at Subfreezing Temperatures.

Cryo letters·2019
Same author

Fast simultaneous assessment of renal and liver function using polymethine dyes in animal models of chronic and acute organ injury.

Scientific reports·2017
Same author

Hypothermic machine perfusion of rat livers preserves endothelial cell function.

Transplantation proceedings·2005
Same author

Hepatic function in hypothermically stored porcine livers: comparison of hypothermic machine perfusion vs cold storage.

Transplantation proceedings·2005
Same author

Rhabdomyosarcoma of the parotid region occurring in childhood and adolescence. A report from the Intergroup Rhabdomyosarcoma Study Group.

Cancer·2001
Same author

Which patients with microscopic disease and rhabdomyosarcoma experience relapse after therapy? A report from the soft tissue sarcoma committee of the children's oncology group.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2001

Area of Science:

  • * Biochemistry and Metabolism
  • * Physiology
  • * Sepsis Pathophysiology

Background:

  • * Sepsis significantly alters host metabolism, impacting liver function.
  • * Understanding substrate utilization in sepsis is crucial for therapeutic strategies.

Purpose of the Study:

  • * To investigate the effects of catecholamine stimulation on substrate utilization for gluconeogenesis, ureagenesis, and oxidation in perfused livers from septic rats.
  • * To compare substrate handling in septic versus non-septic (sham-operated) rat livers.

Main Methods:

  • * Isolated perfused rat livers from septic and sham-operated animals.
  • * Perfusion with [14C]lactate and varying concentrations of unlabeled alanine or pyruvate.
  • * Measurement of gluconeogenesis, ureagenesis, and oxygen consumption.

Related Experiment Videos

Main Results:

  • * Alanine addition inhibited gluconeogenesis from lactate in septic livers.
  • * Alanine stimulated oxygen consumption via oxidation in sham livers, but lactate oxidation dominated in septic livers with phenylephrine.
  • * Urea production increased with alanine in septic livers, but phenylephrine inhibited ureagenesis in septic livers, unlike sham livers.

Conclusions:

  • * Septic rat livers exhibit fundamental differences in handling nitrogenous and non-nitrogenous gluconeogenic substrates compared to sham livers.
  • * Catecholamine stimulation further modifies substrate utilization patterns in sepsis.
  • * These metabolic alterations in sepsis have significant implications for liver function and overall host response.