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Related Experiment Videos

18:1 n7 fatty acids inhibit growth and decrease inositol phosphate release in HT-29 cells compared to n9 fatty acids

A B Awad1, T Herrmann, C S Fink

  • 1Nutrition Program, State University of New York at Buffalo 14214, USA.

Cancer Letters
|May 4, 1995
PubMed
Summary

Trans and cis vaccenic acids (18:1, n7) inhibited colon cancer cell growth, while 18:1, n9 isomers had no effect. Vaccenic acids impacted the phosphoinositide cycle, suggesting a mechanism for growth inhibition.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Cancer Research

Background:

  • Trans fatty acids are implicated in chronic diseases, including cancer.
  • Understanding the specific effects of different fatty acid isomers on cancer cells is crucial for targeted interventions.
  • The phosphoinositide cycle plays a role in cellular signaling and proliferation.

Purpose of the Study:

  • To investigate the impact of various 18:1 fatty acid isomers (positional and geometrical) compared to 18:0 on HT-29 human colon cancer cell growth.
  • To analyze the effects of these fatty acids on the phosphoinositide cycle in colon cancer cells.
  • To explore potential mechanisms linking fatty acid supplementation to cancer cell behavior.

Main Methods:

  • HT-29 cells were supplemented with stearic acid (18:0), elaidic acid (18:1, n9, trans), oleic acid (18:1, n9, cis), vaccenic acid (18:1, n7, cis), or trans-vaccenic acid (18:1, n7, trans).

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  • Cell growth was quantified by cell counting over a 9-day period.
  • The phosphoinositide cycle was assessed by measuring inositol phosphate production under basal and stimulated conditions.

    • Main Results:

    • Cis and trans n7 fatty acids (vaccenic acids) inhibited HT-29 cell growth by 11% and 23%, respectively, compared to the control (18:0).
    • 18:1, n9 isomers (elaidic and oleic acids) did not affect tumor growth.
    • All 18:1 isomers increased inositol phosphate (IP) and IP2 production, with a notable increase in total IP production for 18:1, n9 isomers. Trans fatty acid supplementation led to lower phosphoinositide hydrolysis than cis forms.

    Conclusions:

    • Vaccenic acids (cis and trans 18:1, n7) exhibit inhibitory effects on human colon cancer cell growth.
    • The observed growth inhibition may be mediated by alterations in the phosphoinositide cycle.
    • Incorporation of vaccenic acids into membrane lipids could influence their anti-cancer effects.