Related Experiment Videos
Polarized secretion of interleukin-8 by human mesothelial cells: a role in neutrophil migration
A M Zeillemaker1, F P Mul, A A Hoynck van Papendrecht
1Department of Surgery, Diakonessen Hospital, Utrecht, The Netherlands.
Abstract:
We investigated the role of human mesothelium in an in vitro model of peritonitis with emphasis on the secretion of the neutrophil chemoattractant interleukin-8 (IL-8) and the migration of polymorphonuclear leucocytes (PMN) across monolayers of peritoneal mesothelial cells. PMN showed minimal migration across non-activated mesothelial monolayers (< 2%). However, migration was induced after mesothelial cell activation by IL-1 beta (24%) and this induced migration was significantly blocked by antibodies against IL-8 (63% inhibition; P < or = 0.01). IL-1 beta-activated mesothelial monolayers were shown to secrete IL-8 in a polarized way, which was preferentially oriented towards the apical side of the monolayer. Our results indicate that the influx of PMN into the peritoneal cavity is, at least in part, controlled by the mesothelial cell layer of the peritoneal membrane.
Insights
Human mesothelium controls neutrophil influx during peritonitis. Activated mesothelial cells secrete interleukin-8 (IL-8), promoting polymorphonuclear leucocyte (PMN) migration, which is blocked by IL-8 antibodies.
Area of Science:
- Immunology
- Cell Biology
- Peritoneal Research
Background:
- The peritoneal mesothelium forms a critical barrier in the abdominal cavity.
- Understanding its role in inflammatory responses, particularly peritonitis, is crucial for therapeutic development.
Purpose of the Study:
- To investigate the role of human peritoneal mesothelial cells in peritonitis.
- To examine the secretion of interleukin-8 (IL-8) and the migration of polymorphonuclear leucocytes (PMN) across mesothelial monolayers.
Main Methods:
- Utilized an in vitro model of peritonitis using human peritoneal mesothelial cell monolayers.
- Assessed PMN migration across non-activated and IL-1 beta-activated mesothelial cells.
- Measured IL-8 secretion and the effect of IL-8 antibodies on PMN migration.
Main Results:
- PMN migration across non-activated mesothelial monolayers was minimal (< 2%).
- IL-1 beta activation significantly increased PMN migration (24%).
- Antibodies against IL-8 blocked this induced migration by 63% (P < 0.01).
- Activated mesothelial cells exhibited polarized IL-8 secretion towards the apical side.
Conclusions:
- Human peritoneal mesothelial cells play a significant role in controlling PMN influx into the peritoneal cavity during peritonitis.
- IL-8 secretion by activated mesothelial cells is a key mechanism driving PMN migration.
- The mesothelium acts as a dynamic regulator of peritoneal inflammation.