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Related Experiment Videos

A new B18 sequence (B*1802) from Asian individuals

L Lin1, K Tokunaga, Y Ishikawa

  • 1Department of Research, Japanese Red Cross Central Blood Center, Tokyo.

Human Immunology
|January 1, 1995
PubMed
Summary

A novel Human Leukocyte Antigen B18 allele (HLA-B1802) was identified in a Thai individual, differing from the previously known HLA-B1801 allele. This discovery highlights genetic diversity within the HLA-B18 group.

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Area of Science:

  • Immunogenetics
  • Molecular Biology
  • Human Genetics

Background:

  • Human Leukocyte Antigen (HLA) genes are crucial for immune response and transplantation.
  • HLA allele diversity is essential for understanding population genetics and disease susceptibility.
  • Previous characterization of HLA-B18 alleles did not fully capture the genetic variations in Asian populations.

Purpose of the Study:

  • To sequence and characterize a newly identified HLA-B18 allele from a Thai individual.
  • To compare the nucleotide sequence of the new allele with the established HLA-B1801 allele.
  • To investigate the frequency and associated HLA alleles of the new allele in an Asian cohort.

Main Methods:

  • Nucleotide sequencing of the novel HLA-B18 allele.

Related Experiment Videos

  • Bioinformatic comparison of the new allele's sequence with HLA-B1801.
  • Polymerase Chain Reaction Sequence-Specific Oligonucleotide (PCR-SSO) typing for allele identification.
  • Analysis of associated HLA class I and class II alleles.

    • Main Results:

    • A new HLA-B18 allele, designated HLA-B1802, was identified and sequenced.
    • HLA-B1802 exhibits distinct nucleotide sequences compared to HLA-B1801, with three nucleotide changes in exon 3.
    • Two substitutions at codon 97 result in an amino acid change from arginine to asparagine (97Asn) in HLA-B1802.
    • A silent mutation at codon 99 was also observed in HLA-B1802.
    • PCR-SSO analysis confirmed the presence of HLA-B1802 in three out of 18 examined Asian samples.
    • These three samples carrying HLA-B1802 also shared HLA-Cw7, HLA-DR12, and HLA-DQ7.

    Conclusions:

    • The identification of HLA-B1802 expands the known allelic repertoire of HLA-B18.
    • The observed amino acid change at residue 97 may have implications for peptide binding and immune recognition.
    • The prevalence of HLA-B1802 in the studied Asian population suggests its significance in regional immunogenetics.
    • Further studies are warranted to explore the functional and clinical relevance of HLA-B1802 and its associated alleles.