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Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture
M Amicosante1, L Richeldi, G Trenti
1Dipartimento di Scienze Mediche, Oncologiche e Radiologiche, Università di Modena, Italy.
Journal of Clinical Microbiology
|March 1, 1995
Abstract:
Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).