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Nonradioactive PCR-enzyme-linked immunosorbent assay method for detection of human cytomegalovirus DNA
R D Allen1, P E Pellett, J A Stewart
1Viral Exanthems and Herpesvirus Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Journal of Clinical Microbiology
|March 1, 1995
Abstract:
We developed a rapid, sensitive, and specific PCR-based assay for human cytomegalovirus (HCMV). The assay includes primer and probe sequences derived from conserved HCMV nucleotide sequences and nonradioactive hybridization-confirmation. The assay detected between 10 and 100 viral genomes. All HCMV clinical isolates tested (39 of 39) gave positive reactions.