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Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
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Published on: August 13, 2013

Naive CD28-deficient T cells can initiate but not sustain an in vitro antigen-specific immune response

P J Lucas1, I Negishi, K Nakayama

  • 1Howard Hughes Medical Institute, Washington School of Medicine, St. Louis, MO 63110, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|June 1, 1995
PubMed

Naive T cells require an Ag-specific signal, as well as a costimulatory signal to mount a primary Ag-specific response. Because of their low precursor frequency, it has been difficult to study costimulatory requirements of these Ag-specific T cells. We have generated a CD28-deficient mouse that has been bred to a TCR transgenic (Tg) mouse to better study the function of CD28 during CD4+ T cell responses to Ag. In the absence of CD28, naive TCR Tg T cells responded vigorously to peptide, but responded poorly to mitogen activation. Comparison of activation-induced cell-surface molecules, including CD25, CD44, CD69, and CD71, showed no significant differences between CD28+ and CD28- TCR Tg T cells during the first 24 to 48 h after Ag stimulation. Despite relatively normal surface phenotype and normal proliferative response to Ag, CD28- T cells produced little IL-2, had a decreased sensitivity to lower Ag concentrations, and were unable to maintain their proliferative response. These results suggest that naive T cells are able to utilize other costimulatory signals to initiate a primary Ag-specific response, but require CD28 for optimal, sustained proliferation.

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