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Related Experiment Videos

Developing neurons use a putative pioneer's peripheral arbor to establish their terminal fields

W B Gan1, E R Macagno

  • 1Department of Biological Sciences, Columbia University, New York, New York 10027, USA.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|May 1, 1995
PubMed
Summary

Leech pioneer neurons, like the pressure-sensitive (PD) neuron, provide essential guidance for developing neurons. Their peripheral arbors act as a necessary and sufficient template for forming normal terminal fields in follower neurons.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Neuroanatomy

Background:

  • Pioneer neurons guide axonal outgrowth in developing nervous systems.
  • The role of pioneer neurons in forming terminal fields of follower neurons is not well understood.

Purpose of the Study:

  • To investigate the role of the leech pressure-sensitive (PD) neuron in the formation of peripheral arbors by later developing neurons.
  • To determine if the PD neuron's arbor serves as a template for follower neuron terminal field development.

Main Methods:

  • Ablation of PD neurons in leeches.
  • Observation of the growth and arborization of AP motor neurons and TD sensory neurons.
  • Manipulation of PD neuron minor axon outgrowth.

Main Results:

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  • Later developing neurons precisely followed the PD neuron's major axon and peripheral arbor.
  • Ablation of PD neurons resulted in reduced and abnormal terminal arbors in follower neurons.
  • Follower neuron arbors grew along novel PD neuron branches when induced, demonstrating template function.

Conclusions:

  • The peripheral arbor of the PD neuron is crucial for the normal development of terminal fields in specific follower neurons.
  • The PD neuron's arbor acts as both a necessary and sufficient template for follower neuron arbor formation.