[Amperometric enzyme biosensor with a glucose oxidase-polyaniline membrane]

Area of Science:

• Electrochemistry
• Biomaterials Science
• Analytical Chemistry

Context:

• Development of electrochemical biosensors for glucose detection is crucial for diagnostics.
• Enzyme immobilization into polymer matrices is a key strategy for biosensor stability and performance.
• Polyaniline films offer a versatile platform for entrapping biomolecules due to their electrochemical properties.

Purpose:

• To fabricate an amperometric glucose biosensor by electrochemical polymerization of aniline onto gold electrodes.
• To immobilize glucose oxidase enzyme within the polyaniline film for glucose detection.
• To determine the optimal conditions for preparing the polyaniline-glucose oxidase biosensor and evaluate its performance.

Summary:

• An amperometric glucose biosensor was successfully fabricated via electrochemical polymerization of aniline in the presence of glucose oxidase on gold electrodes.
• The glucose oxidase enzyme was effectively entrapped within the resulting polyaniline film, acting as a catalyst for glucose hydrolysis.
• The biosensor demonstrated amperometric determination of glucose in the concentration range of 10⁻⁴ M to 2 x 10⁻² M, with linearity from 2 x 10⁻⁴ M to 6 x 10⁻³ M.

Impact:

• This electrochemical synthesis method provides a high-technologic route for creating robust polyaniline-enzyme thin films.
• The developed technique facilitates the fabrication of various microbiosensors and multisensors within a continuous technological cycle.
• This advancement contributes to the field of electrochemical sensing with potential applications in glucose monitoring and diagnostics.