Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analytical problems encountered with NIOSH method 5521 for total isocyanates

R J Key-Schwartz1

  • 1Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Cincinnati, Ohio 45226, USA.

American Industrial Hygiene Association Journal
|May 1, 1995
PubMed
Summary

National Institute for Occupational Safety and Health Method 5521 faced challenges identifying isocyanate oligomers in air samples. Supplementing with UV spectra from a photodiode array detector improved identification, questioning the original method's sufficiency.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Determination of airborne isocyanate exposure: considerations in method selection.

AIHAJ : a journal for the science of occupational and environmental health and safety·2000
Same author

An approach to area sampling and analysis for total isocyanates in workplace air.

American Industrial Hygiene Association journal·1999
See all related articles

Area of Science:

  • Occupational Health and Safety
  • Analytical Chemistry
  • Environmental Monitoring

Background:

  • National Institute for Occupational Safety and Health (NIOSH) Method 5521 is used for analyzing total isocyanates in air.
  • Challenges exist in identifying specific oligomeric isocyanate species using this method.
  • Common issues include false negative and false positive results in chromatographic analysis.

Purpose of the Study:

  • To address difficulties in identifying oligomeric isocyanate species using NIOSH Method 5521.
  • To evaluate the effectiveness of supplementing the method's ratio criterion with additional spectral data.
  • To determine if the existing ratio criterion is adequate for qualitative identification of isocyanate oligomers.

Main Methods:

  • Analysis of total isocyanates in air samples using NIOSH Method 5521.

Related Experiment Videos

  • High-performance liquid chromatography (HPLC) with UV detection.
  • Supplementation of the method's ratio criterion with complete UV absorption spectra from a photodiode array (PDA) detector.

    • Main Results:

    • A false negative response was observed in most field samples during HPLC analysis.
    • An anomalous peak caused false positive results in some field blanks and samples.
    • Supplementing with PDA UV spectral data successfully identified the problematic peaks, including an oligomeric isocyanate species.

    Conclusions:

    • The ratio criterion of NIOSH Method 5521 alone may be insufficient for the qualitative identification of isocyanate oligomers.
    • Utilizing complete UV absorption spectra from a PDA detector enhances the accuracy of isocyanate analysis.
    • Further validation is needed to confirm the reliability of NIOSH Method 5521 for oligomeric isocyanate identification.