Mapping of stimulus features and meaning in gerbil auditory cortex with 2-deoxyglucose and c-Fos antibodies

The basic functional organization of gerbil auditory cortex was previously mapped with unit recording of best frequency and with the fluoro-2-deoxyglucose mapping (FDG) technique. Among at least seven subfields in this cortex the primary auditory cortex (AI) and the anterior auditory field (AAF) showed prominent tonotopic organization with parallel dorsoventral iso-frequency contours (electrophysiology) in correspondence to FDG labelling of frequency band laminae. In an approach to mechanisms of learning aversive tone conditioning paradigms were found to reshape frequency receptive fields of single units in AI and also produced spatial shifts of tone representation in the tonotopic maps of AI and AAF. Both results suggest that spectral features as well as aspects of behavioural meaning of sounds may be represented even in primary auditory cortex. General meaningfulness in terms of occurrence of novel and salient stimuli may be reflected by expression of immediate early genes. Mapping with an antibody against the immediate early gene product c-Fos was performed in order to identify the spatial distribution of neurons in auditory cortex which change metabolism as a result of stimulation with auditory signals in a new environment. Very short e.g. less than 3 min repetitive stimulation with a tone led to frequency-specific columnar expression of c-Fos in AI and to spare non-tonotopic expression in other fields. Longer stimulation or longer aversive conditioning with the same tone led to spreading of expression, i.e. to accessory non-tonotopic labelling in AI and other fields, particularly pronounced in the output layers V and VI. It is assumed that this spreading relates to the formation of output schemes from auditory cortex in terms of implicit behavioural meaning of stimuli.