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[Detection of polynucleotide fragments using hybridization with probes labelled with digoxigenin]
R Cîrciumaru1, G Anton, M Stoian
1Institut de Virologie Stefan S. Nicolau, Bucarest, Roumanie.
Revue Roumaine De Virologie (Bucharest, Romania : 1990)
|January 1, 1994
Abstract:
A new nonradioactive system for labeling molecular probes, using digoxygenine, was studied. Detection of ADN-ADN type hybrids formed on a nylon membrane was realised photochemically or by ELISA, dot-blot or Southern-blot. The high sensitivity and specificity of this new system allow to use it for detection of amounts of ADN as small as 5 ng.