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Decrease in CRE binding activity by chronic morphine administration in mouse brain

M Ikemoto1, T Osugi, X B Wang

  • 1Department of Pharmacology I, Osaka University Medical School, Japan.

Neuroreport
|January 26, 1995
PubMed
Summary
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Chronic morphine treatment reduces cAMP response element (CRE) binding activity in key brain regions, indicating long-term changes in gene expression related to opiate addiction.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Opiate addiction is linked to alterations in gene transcription within the brain.
  • Understanding these transcriptional changes is crucial for developing effective addiction treatments.

Purpose of the Study:

  • To investigate the impact of chronic morphine treatment on DNA binding activity of transcription factors in the mouse brain.
  • To introduce and validate a novel method, in situ DNA-protein binding (ISDB), for assessing these changes.

Main Methods:

  • Development and application of the in situ DNA-protein binding (ISDB) technique.
  • Administering chronic morphine to mice and analyzing brain tissue from specific regions (amygdala, thalamus, cerebral cortex, hypothalamus).
  • Measuring changes in cAMP response element (CRE) binding activity post-morphine treatment and withdrawal.

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Main Results:

  • Chronic morphine treatment significantly decreased cAMP response element (CRE) binding activity.
  • This reduction was observed in multiple brain regions, including the amygdala complex, thalamus, cerebral cortex, and hypothalamus.
  • The observed decrease in CRE binding activity persisted for at least 14 days after morphine cessation.

Conclusions:

  • Chronic morphine exposure induces long-lasting alterations in DNA binding activity of transcription factors in the brain.
  • These changes suggest a sustained impact on cAMP-mediated gene expression pathways, potentially contributing to the persistence of opiate addiction.
  • The ISDB method provides a valuable tool for studying dynamic changes in transcription factor activity in vivo.