Liquid chromatographic method for rapid determination of total avermectin B1 and 8,9-Z-avermectin B1 residues in...

A liquid chromatographic method has been developed and validated for the rapid determination of avermectin B1 and 8,9-Z-avermectin B1 residues in apples. The avermectins are extracted from the crop matrix with an acetonitrile-water-hexane mixture; the extract is cleaned up on an aminopropyl solid-phase extraction column. The avermectins are derivatized with trifluoroacetic anhydride and analyzed by reversed-phase liquid chromatography with fluorescence detection. Recoveries of avermectins from apples fortified with about 2-77 ppb avermectin B1a or 2-27 ppb 8,9-Z-avermectin B1a averaged 85%. The limit of quantitation is 2 ppb (signal-to-noise [S/N] ratio, 12) and the limit of detection is 1 ppb (S/N ratio, 6) for each analyte. The assay is a simple, rapid, and sensitive method for monitoring the total amount of avermectin residues in apples.