Related Experiment Video

Updated: Aug 8, 2026

FIBS-enabled Noninvasive Metabolic Profiling
09:16

FIBS-enabled Noninvasive Metabolic Profiling

Published on: February 3, 2014

[Fructose-2,6-bisphosphate system and experimental states]

B F Korovkin, N F Beliaeva, L N Viktorova

    Vestnik Rossiiskoi Akademii Meditsinskikh Nauk
    |January 1, 1995
    PubMed

    More Related Videos

    Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry08:06

    Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

    Published on: October 25, 2019

    Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems14:42

    Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

    Published on: September 23, 2021

    Related Concept Videos

    Energy-requiring Steps of Glycolysis01:20

    Energy-requiring Steps of Glycolysis

    Glucose is the source of nearly all energy used by organisms. The first step of converting glucose into usable energy is called glycolysis. Glycolysis occurs in the cytosol of the cell over two phases: an energy-requiring phase and an energy-releasing phase. Over the first three steps, glucose is converted into different forms and attached to two phosphate groups donated by two ATP molecules, resulting in an unstable sugar. In the next two stages, the unstable sugar splits into two sugar...
    Glycolysis: Preparatory Phase01:21

    Glycolysis: Preparatory Phase

    In cellular metabolism (the complete breakdown of glucose to extract energy),  glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...
    ATP Energy Storage and Release01:31

    ATP Energy Storage and Release

    ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
    One example of energy coupling using ATP involves a...
    Glycolysis: Pay-off Phase01:25

    Glycolysis: Pay-off Phase

    So far, glycolysis has cost the cell two ATP molecules and produced two small, three-carbon sugar molecules. These molecules will proceed through the second half of the pathway, and sufficient energy will be extracted to pay back the two ATP molecules used as an initial investment and produce a profit for the cell of two additional ATP molecules and two even higher-energy NADH molecules.
    Step 1 - 5: Glycolysis Preparatory Phase
    The first phase of glycolysis has 5 steps where the glucose is...
    Other Glycolytic Pathways01:24

    Other Glycolytic Pathways

    The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
    Biosynthesis of Polysaccharides01:26

    Biosynthesis of Polysaccharides

    Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...