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Related Experiment Video

Updated: Jul 12, 2026

Irrelevant Stimuli and Action Control: Analyzing the Influence of Ignored Stimuli via the Distractor-Response Binding Paradigm

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Published on: May 14, 2014

[Search, isolation and study of restrictases]

Vestnik Rossiiskoi Akademii Meditsinskikh Nauk

|January 1, 1995

Summary

Researchers discovered 32 new restriction endonucleases from over 800 microorganisms using a novel toluene microtechnique. These enzymes, including CsiBI, are valuable tools for molecular genetics and genome analysis.

Area of Science:

Molecular Biology
Enzymology
Microbiology

Background:

Restriction endonucleases are crucial tools in molecular biology for DNA manipulation.
Identifying novel enzymes with unique recognition sites expands the toolkit for genetic engineering.

Purpose of the Study:

To discover and characterize new Class II restriction endonucleases from a diverse range of microorganisms.
To evaluate the potential applications of these novel enzymes in molecular genetic studies.

Main Methods:

Screening of over 800 microbial strains using a sensitive toluene microtechnique to detect endonuclease activity.
Purification of identified restriction endonucleases to ensure high purity and absence of contaminating nucleases.
Characterization of enzyme specificity, including the identification of recognition sites for novel enzymes like CsiBI.

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RBDT: A Computerized Task System based in Transposition for the Continuous Analysis of Relational Behavior Dynamics in Humans

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Related Experiment Videos

Last Updated: Jul 12, 2026

Irrelevant Stimuli and Action Control: Analyzing the Influence of Ignored Stimuli via the Distractor-Response Binding Paradigm

Irrelevant Stimuli and Action Control: Analyzing the Influence of Ignored Stimuli via the Distractor-Response Binding Paradigm

Published on: May 14, 2014

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

RBDT: A Computerized Task System based in Transposition for the Continuous Analysis of Relational Behavior Dynamics in Humans

RBDT: A Computerized Task System based in Transposition for the Continuous Analysis of Relational Behavior Dynamics in Humans

Published on: July 17, 2021

Main Results:

Identification of 72 microbial strains exhibiting site-specific endonuclease activity.
Isolation and purification of 32 novel Class II restriction endonucleases.
Discovery of CsiBI from Corynebacterium species, a novel enzyme recognizing an 8-nucleotide sequence (GGGGGGGG), useful for generating large genomic fragments.

Conclusions:

The toluene microtechnique is effective for discovering new restriction enzymes.
The identified novel restriction endonucleases, particularly CsiBI, offer significant potential for advanced molecular genetic applications, including large-scale genome analysis.