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Determination of fumonisins B1, B2, B3 and B4 by high-performance liquid chromatography with evaporative
J G Wilkes1, J B Sutherland, M I Churchwell
1Division of Chemistry, National Center for Toxicological Research, United States Food and Drug Administration, Jefferson, AR 72079, USA.
Journal of Chromatography. A
|March 31, 1995
Abstract:
Fumonisins B1, B2, B3 and B4 (FB1-FB4), a group of mycotoxins produced by the fungus Fusarium moniliforme, were separated by HPLC using an analytical-scale, base-deactivated C8 column and a gradient of trifluoroacetic acid buffer (pH 2.7) and acetonitrile. An evaporative light-scattering detector was used to detect the fumonisin peaks. A semi-preparative-scale, base-deactivated C8 column with a 1:14 mobile phase split facilitated the purification of analytical standards of FB.